How to Design Your Own Primers and Probes for Real-Time PCR with Primer Express Software Version 3.0
If you are looking for a software that can help you design your own primers and probes for gene quantitation and allelic discrimination (SNP) real-time PCR applications, you may want to check out Primer Express Software Version 3.0. This software allows you to use TaqMan and SYBR Green I dye chemistries, which are compatible with various real-time PCR systems from Thermo Fisher Scientific. In this article, we will show you how to download and use Primer Express Software Version 3.0 to create your own custom assays.
Primer Express Software Version 3.0 Free Download
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What is Primer Express Software Version 3.0?
Primer Express Software Version 3.0 is a software that enables individual oligonucleotide design for real-time PCR applications[^1^]. It supports assays based on TaqMan and SYBR Green I dye chemistries, which are widely used for gene quantitation and allelic discrimination (SNP) studies[^1^]. Primer Express Software Version 3.0 provides design flexibility, ease-of-use, and requires minimal optimization[^1^]. It also provides robust assay performance when used in accordance with Assay Development Guidelines[^1^].
Primer Express Software Version 3.0 is developed specifically for use with StepOne, StepOnePlus, 7300, 7500, 7500 Fast, 7900HT, ViiA 7, and QuantStudio real-time PCR systems from Thermo Fisher Scientific[^1^] [^2^]. These systems offer high sensitivity, accuracy, and reproducibility for various real-time PCR applications. Primer Express Software Version 3.0 provides customized application-specific documents for absoluteârelative quantitation and allelic discrimination[^1^].
How to Download Primer Express Software Version 3.0?
To download Primer Express Software Version 3.0, you need to purchase a license key from Thermo Fisher Scientific[^2^]. You can choose between a single-user license or a five-user license depending on your needs. The price of the license key varies depending on your location and currency[^2^]. Once you have purchased the license key, you can download the software from the Thermo Fisher Scientific website[^1^]. You will also receive an email with the download link and instructions on how to install and activate the software.
How to Use Primer Express Software Version 3.0?
Primer Express Software Version 3.0 is easy to learn and use. It includes an algorithm optimized specifically for use with TaqMan-based reagents, recommended primerâprobe concentrations, and default thermal cycling conditions[^2^]. Primer Express Software Version 3.0 also supports assays based on the SYBR Green I dye[^2^]. This minimizes the need for assay optimization and streamlines the design process.
To use Primer Express Software Version 3.0, you need to import (or copy and paste) the sequence of your target gene or SNP into the software[^2^]. You can then choose either TaqMan or TaqMan MGB assays as your chemistry option[^2^]. The software will automatically design your primers and probes based on the sequence and the chemistry parameters[^2^]. You can also design them manually if you prefer or if you already know some of the oligonucleotides[^2^]. The software will display the results in a table format with various information such as primerâprobe sequences, lengths, melting temperatures, GC contents, amplicon sizes, etc.[^2^]. You can also view the graphical representation of your primers and probes on the sequence map[^2^]. You can then export or print your results for further analysis or ordering.
Conclusion
Primer Express Software Version 3.0 is a powerful tool that can help you design your own primers and probes for real-time PCR applications using TaqMan and SYBR Green I dye chemistries. It is compatible with various real-time PCR systems from Thermo Fisher Scientific and provides robust assay performance when used in accordance with Assay Development Guidelines. To download Primer Express Software Version 3.0, you 29c81ba772